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Activation of Human T and B Cells by Rabbit Anti‐Human β 2 ‐Microglobulin
Author(s) -
RINGDÉN O.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00217.x
Subject(s) - phytohaemagglutinin , stimulation , microbiology and biotechnology , beta (programming language) , beta 2 microglobulin , dna synthesis , spleen , biology , immunology , endocrinology , dna , biochemistry , programming language , computer science
Rabbit anti‐human β 2 ‐microglobulin (anti‐β 2 m) increased the highest DNA synthesis in un‐separated lymphocytes or artificially composed mixtures enriched in T and B cells. In enriched T and B cells no or low stimulation was seen. The maximal response by IgG anti‐β 2 m was seen in proportions of enriched T/B cells, being 3:1 for blood lymphocytes and 1:1 for spleen cells, which are the same as the physiological proportions of T and B cells in these lymphoid organs. Whereas unseparated lymphocytes gave a peat response on day 3, enriched B cells had a peak response on day 6. Fab anti‐β 2 m did not activate enriched T cells but increased DNA synthesis in enriched B cells to about the same extent as unseparated lymphocytes and mixtures of enriched T and B cells. The proportion of sheep erythrocyte rosette‐forming cells (E‐RFC) decreased after stimulation by anti‐β 2 m and increased after stimulation by phytohaemagglutinin. However, as revealed by autoradiography, a proportion of lymphocytes activated by anti‐β 2 m were E‐RFC and this proportion increased with increasing stimulation by anti‐β 2 m. DNA synthesis induced by anti‐β 2 m was unchanged for spleen cells and slightly decreased for blood lymphocytes when phagocytic cells were removed by iron treatment. Supernatants from lymphocytes activated by anti‐β 2 m only induced low DNA synthesis in enriched T or B cells. Experiments with mitomycin treated cells indicate that cooperation and close contact between T and B cells are needed for activation by IgG anti‐β 2 m. T cells are needed for B cell activation by anti‐β 2 m and B cells are required for T cell activation to occur.