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Synthesis of Secretory Component by Rat Hepatocytes in Culture
Author(s) -
ZEVENBERGEN J. L.,
MAY C.,
WANSON J. C.,
VAERMAN J. P.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00213.x
Subject(s) - secretory component , antiserum , in vitro , radioimmunoassay , precipitin , secretion , component (thermodynamics) , secretory protein , hepatocyte , chemistry , biology , biochemistry , antibody , immunology , physics , thermodynamics
Normal rat hepatocytes were isolated and cultivated in vitro. Synthesis of secretory component was demonstrated by its accumulation in the culture medium, as measured by radioimmunoassay; by incorporation of 14 C‐leucine in the protein specifically precipitated with anti‐secretory component antiserum; and by a positive precipitin reaction of concentrated culture medium with the same antiserum. The results explain the high levels of secretory component found in rat bile and render plausible a mechanism of hepatic IgA transfer involving secretory component as the hepatocyte membrane receptor for polymeric IgA.