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d ‐Penicillamine in Vivo Enhances Lymphocyte DN A Synthesis: Role of Macrophages
Author(s) -
BINDERUP L.,
BRAMM E.,
ARRIGONIMARTELLI E.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00204.x
Subject(s) - penicillamine , concanavalin a , spleen , in vivo , thymidine , in vitro , macrophage , lymph node , lymphocyte , chemistry , medicine , endocrinology , lymphatic system , pharmacology , immunology , biology , biochemistry , microbiology and biotechnology
Administration of d ‐penicillamine (50 mg/kg/day orally) for 4 days increased the uptake of 3 H‐thymidine ( 3 H‐TdR) in unstimulated and concanavalin ‐A‐stimulated unseparated lymph node and spleen cells from Lewis rats. Increased 3 H‐TdR incorporation was also found in cultures depleted of adherent cells. d ‐Penicillamine treatment did not increase the incorporation of 3 H‐TdR in lymph node and spleen cells from rats concomitantly treated with the selective macro‐phage toxin silica. in contrast, treatment with d ‐penicillamine during the last 4 days of silica treatment sometimes resulted in a marked decrease in 3 H‐TdR incorporation. It is suggested that d ‐penicillamine treatment in vivo is able to enhance the responsiveness of the lymphocytes, dependent on the presence of functionally intact macrophages. The increased response vanished after 2‐3 weeks, even with continuous administration of d ‐penicillamine.