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Suppressor Activity of T Cells Bearing Fc Receptors for IgG on Lymphoid Colony Formation
Author(s) -
CAICA G. W.,
PISTOIA V.,
GHIO R.,
MINGARI M. C.,
MORETTA L.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00099.x
Subject(s) - phytohaemagglutinin , receptor , suppressor , immune system , stimulation , microbiology and biotechnology , lymphatic system , biology , immunology , t cell , chemistry , endocrinology , biochemistry , gene
Human T lymphocytes, upon phytohaemagglutinin stimulation, are able to form colonies in semisolid media. Peripheral T cells bearing Fc receptors (T G ) were studied for their possible regulatory activity on lymphoid colony development. Although no substantial differences were observed between the cloning efficiency of unfractionated T cells (thus including T G cells) and T cells depleted of T G , a sharp suppression of colony formation occurred when positively selected T G cells were readded to T G ‐depleted suspensions. Therefore, T G suppressor activity seems to be strictly dependent upon cell interaction with ox IgG immune complexes used for T G cell isolation. Different experimental approaches failed to demonstrate, although did not exclude, that suppression in this system is mediated by soluble factors, γ‐irradiation of T G cells abrogated their suppressor capacity.