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Effect of Cyclosporin A on the Generation of Cytotoxic T Lymphocytes in Mouse Mixed Lymphocyte Culture
Author(s) -
NEMLANDER A.,
HÄYRY P.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00096.x
Subject(s) - cytotoxic t cell , microgram , lymphocyte , immune system , immunology , drug , spleen , growth inhibition , biology , t lymphocyte , pharmacology , cell culture , chemistry , microbiology and biotechnology , in vitro , biochemistry , genetics
Cyclosporin A inhibited at equal concentrations both the proliferate response and the generation of cytotoxic T lymphocytes in one‐way mouse spleen cell mixed lymphocyte culture. The 50% inhibitory concentration was in all experiments 10 −2 to 10 −1 μg/ml. The inhibition was directly proportional to how early the drug was added to the culture: a complete inhibition of both responses was obtained if the drug was added on day.3, and a partial inhibition if added on day 4 of culture. Cytological analysis of the cultured cells demonstrated that resting lymphocytes were not damaged at 100 1000‐fold concentrations of the drug giving complete inhibition of the blastogenic response. The results suggest that cyclosporin A is most effective if present throughout the induction phase of the immune response.