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Differential Role of Zn 2+ in Antigen‐ and Mitogen‐induced Lymphokine Production
Author(s) -
BENDTZEN K.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00095.x
Subject(s) - lymphokine , phytohaemagglutinin , in vitro , antigen , chemistry , peripheral blood mononuclear cell , mitogen activated protein kinase , tuberculin , microbiology and biotechnology , biology , immunology , biochemistry , medicine , tuberculosis , pathology
The effect on human lymphokine production in vitro of phenanthroline, a Zn 2+ ‐chelating agent and an inhibitor of carboxypeptidases A and B, was tested. The elaboration of leucocyte migration inhibitory factor (LIF) by tuberculin‐sensitized mononuclear cells stimulated with the specific antigen was reduced in a dose‐dependent manner, an effect completely restored by addition of excess Zn 2+ . In contrast, phenanthroline did not affect LIF production by mono‐nuclear cells activated nonspecifically by phytohaemagglutinin. It is hypothesized that the presence of a Zn 2+ ‐dependent molecule, possibly a carboxypeptidase, may be necessary for antigen‐ but not for mitogen‐induced lymphokine production.