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Dynamics of the Interaction between Entamoeba histolytica and Components of the Immune Response
Author(s) -
AUSTKETTIS A.,
SUNDQVIST K.G.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00088.x
Subject(s) - amoeba (genus) , entamoeba histolytica , antibody , redistribution (election) , antigen , biology , chemistry , microbiology and biotechnology , immunology , politics , political science , law
Trophozoites of Entamoeba histolytica redistributed specific antibodies reacting to antigens on their surface. The presence of a second layer of anti‐antibodies enhanced the redistribution of anti‐amoeba antibodies but was not a prerequisite for this process. Microfluorometric measurements of the fluorescence intensity in different areas of the cell surface provided objective evidence for the antibody‐induced redistribution. In order to distinguish surface‐bound and internalized anti‐amoeba antibodies, the trophozoites were fixed in PFA. This fixation made the cells impermeable to antibodies. Thus, after fixation in PFA at different times during the redistribution process surface‐bound anti‐amoeba antibodies labelled with one fluorochrome could be detected with anti‐antibodies labelled with another fluorochrome. This approach showed that the amoeba, in addition to the redistribution, also internalized anti‐amoeba antibodies.