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Production and Main Characteristics of a Fetal Calf Serum‐specific Cell Line that Induces T and B Cell Differentiation
Author(s) -
RUBIN B.,
COOLEY M. A.,
BORGNE DE KAOUEL C.,
TAYLOR R. B.,
GOLSTEIN P.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00084.x
Subject(s) - spleen , concanavalin a , cytotoxic t cell , biology , ctl* , immunology , thymocyte , cell culture , splenocyte , microbiology and biotechnology , in vitro , cell , t cell , immune system , biochemistry , genetics
Spleen cells from B6 mice injected with fetal calf serum (FCS) could be kept proliferating as a continuous cell line in vitro provided they were cultured in the presence of irradiated syngeneic spleen cells and FCS. Cells in this cell line showed a strong proliferative response when stimulated with concanavalin A (Con A), and they were able to mediate the following functions: (1) they helped the generation of alloantigen‐specific cytotoxic T lymphocytes (CTL) from thymocyte‐spleen cell mixed lymphocyte cultures (MLC), (2) they induced the generation of CTL from normal syngeneic spleen cells in the absence of allogeneic stimulator cells, and (3) they induced normal spleen cells to differentiate into anti‐sheep erythrocyte (SRBC) plaque‐forming cells (PFC), in the absence of SRBC in the cultures. The use of this cell line (called line 12) may thus provide an interesting approach for the study of cellular and molecular requirements for cell‐cell interactions and for the differentiation of T and B effector functions.