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Production of Human IgM Anti‐D in Tissue Culture by EB‐Virus‐transformed Lymphocytes
Author(s) -
BOYLSTON A. W.,
GARDNER B.,
ANDERSON R. L.,
HUGHESJONES N. C.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00077.x
Subject(s) - lymphoblast , antibody , virus , tissue culture , microbiology and biotechnology , virology , chemistry , biology , cell culture , immunology , in vitro , biochemistry , genetics
Lymphocytes obtained from two donors who were producing anti‐D were transformed with Epstein‐Barr virus and grown in tissue culture. The lymphoblasts from both donors produced anti‐D antibody, which agglutinated Rh‐positive cells in saline. Both antibodies belonged to the IgM class; the concentration of anti‐D in the culture fluid from one of the donors was approximately 250–500 ng/ml and bound firmly to Rh‐positive cells with a functional affinity constant of approximately 1 × 10 9 M −1 .