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Serological and Physicochemical Reactivity of Bovine Erythrocytes before and after Trypsin Treatment
Author(s) -
EDEBO L.,
COOMBS R. R. A.,
BINNS R. M.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00059.x
Subject(s) - hemagglutination , chemistry , antibody , trypsin , antigen , immunoglobulin m , hemagglutination assay , immunoglobulin g , microbiology and biotechnology , biochemistry , immunology , biology , enzyme , titer
On partition in aqueous polymer two‐phase systems containing dextran, poly(ethyleneglycol) (PEG), and PEG substituted with charged or hydrophobia groups ‘inagglutinable’ ox erythrocytes showed more negative surface charge and less hydrophobicity than ‘agglutinable’ ox erythrocytes. Trypsin treatment of the erythrocytes increased the agglutinability, reduced the negative charge, and seemed to increase the liability to hydrophobic interaction. Haemagglutination of highly negatively charged of erythrocytes is almost impossible to accomplish by antibody against the ox erythrocytes alone or against IgG antigen linked to the erythrocytes in passive haemagglutination. Likewise, reverse passive (antiglobulin) haemagglutination cannot be accomplished with these cells when immunoglobulin antigens of moderate molecular size (≥900,000) are used. However, these cells may be agglutinated when a non‐charged carrier such as a bacterium coated with sensitizing antibody (immunoglobulin) is used to bridge the antiglobulin‐coupled erythrocytes in a mixed lattice agglutinate. Such a bridging can also be accomplished by heal aggregation of the immunoglobulin antigen.