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Mitogenic Responsiveness of Human T‐Lymphocyte Subpopulations: Regulation by Suppressive Fc‐Receptor‐bearing T Cells and Influence of Fractionation Procedures
Author(s) -
DILLNERCENTERLIND M.L.,
HELLSTRÖM. U.,
ROBERTSSON E.S.,
HAMMARSTRÖM S.,
PFRLMANN P.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00036.x
Subject(s) - avidity , receptor , biology , microbiology and biotechnology , lymphocyte , t cell , immunology , antigen , biochemistry , immune system
The proliferative response induced by leucoagglutinin (La) in different subpopulations of human I lymphocytes was studied Subpopulations enriched in cells with either high‐ or low‐avidity receptors for sheep erythrocytes (SRBC) (E H .+ , E t + ) were prepared by sequential E‐rosetting. In addition, T lymphocytes prepared by E‐rosetting under optimal conditions (E + ror ) were fractionated on wheat germ agglutin (WGA)‐Sepharose columns, rendering fraction enriched in lymphocytes with either low‐ or high‐avidity receptors for WGA. The T lymphocytes were found to comprise at least three functionally distinct subpopulations, differing with respect to mitogen responsiveness. Cell characterized by high‐avidity receptors for WGA and SRBC were highly responsive to La simulation, regardless of the method used for purification. In contrasts cell with low‐avidity receptors for WGA and probably also for SRBC hut lacking Fe receptors for IgG responded only marginally but were conditioned to respond when subjected to E‐rosetting under optimal conditions. This response was suppressed by lymphocytes with Fc receptors for IgG, which probably also had low‐avidity receptors for WGA and SRBC. The lymphocytes with high‐avidity receptors for WGA and SRBC did not appear to be susceptible to suppression by Fcγ + cells.