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Cooperation of Murine F 1 , T and Parental B Lymphocytes in Rejection of a Xenogeneic Tumour: Adaptive Differentiation of B Lymphocytes?
Author(s) -
MARUŠSIĆ M.,
PERKINS E. H.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00029.x
Subject(s) - cytotoxic t cell , immune system , biology , spleen , antigen , immunology , lymphocyte , bone marrow , transplantation , antibody , t lymphocyte , microbiology and biotechnology , genetics , medicine , in vitro
The primary humoral immune response to rat Yoshida ascites sarcoma (YAS) grown in mice was used to study thymus‐dependent (T) and bone marrow‐derived (B) lymphocyte cooperation. It was shown that B6D2F 1 T lymphocytes that do not cooperate with parental B lymphocytes enabled parental B lymphocytes from B6→B6D2F 1 radiation chimaeras to reject the tumour. However, when the bone marrow cells from B6→B6D2F 1 chimaeras were used to reconstitute parental B6 mice, these B6→B6D2F 1 →B6 mice lost their tolerance to D2 transplantation antigens, and their B lymphocytes were not able to cooperate with B6D2F 1 T lymphocytes. In our search for the reasons for the failure of F 1 T cells to be effective in parental and P→F 1 →P TIR mice, rejection of F 1 T cells was excluded because: (i) immune reactivity in TIR mice was found to he either absent or minimal; (ii) parental TIR mice did not produce any detectable cytotoxic antibodies after an intravenous injection of F 1 splenic T cells; and (iii) YAS rejection was not induced at very high doses of F 1 T cells. However, in a cell transfer system we were able to demonstrate that injection of spleen cells from parental TIR mice could thwart the successful cooperation of transferred F 1 T cells with host B cells. Collectively, these data suggest that the changes of the collaborative potential of parental B cells as achieved in the F 1 environment could be ascribed to ‘adaptive’ differentiation of B lymphocytes. It appears that the differentiation process that has rendered nonsyngeneic chimaeric cells able to cooperate was independent of the exogenous antigen.