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Reactivity of Human Lymphoid and Lymphoblastoid Cells with Peanut Agglutinin: Detection of a Blood Cell Subset which Lacks Detectable Membrane HLA
Author(s) -
BALLET J.J.,
FELLOUS M.,
SHARON N.,
REISNER Y.,
AGRAPART M.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00024.x
Subject(s) - lymphoblast , immunology , human leukocyte antigen , agglutinin , biology , peanut agglutinin , cell , microbiology and biotechnology , antigen , cell culture , genetics , lectin
The number of cells reacting with flurescein‐labelled peanut agglutinin varies widely among human lymphoid organs. By separation techniques, reactive cell from tonsils were characterized as E‐negative, non‐immunoglobulin‐bearing lymphocytes. In peripheral blood, the lectin reacted with a small percentage of lymphoid and monocytoid cells which lacked HLA membrane determinants but expressed the β 2 ‐mieroglobulin antigens. The tatter phenotype was also found on human lymphoblastoid cell lines. Lectin binding thus provides an effective means of characterizing these cell subsets.