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The Accessory Cell Effects of Liver Macrophages in Concanavalin A Stimulation of Rat Spleen Lymphocytes
Author(s) -
MUNTHEKAAS A. C.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00020.x
Subject(s) - concanavalin a , pronase , stimulation , macrophage , spleen , receptor , collagenase , lymphocyte , biology , cell , immunology , chemistry , microbiology and biotechnology , endocrinology , biochemistry , in vitro , trypsin , enzyme
Liver and peritoneal macrophages under similar test conditions behaved in an identical manner with regard to accessory cell effects in the lymphocyte response to concanavalin A. When present in low concentrations (≤3.3%) they stimulate lymphocytes, and when present in high concentration (≥10%) they inhibit lymphocyte proliferation. These two effects are, however, mediated through totally different mechanisms. Stimulation was an early effect, required viable cells, was not affected by enzymatic treatment of macrophages, and was similar to the effect of 2‐mercaptoethanol, allogeneic macrophages, and even non‐macrophages. Inhibition occurred at a later stage of lymphocyte transformation, was sensitive to collagenase and pronase treatment of macrophages, was more specifically due to macrophages, was reduced with allogeneic macrophages. and persisted after freeze‐thawing of macrophages. Removal of Fe receptors or related segments of the surface of macrophages greatly reduced their inhibitory capacity, whereas removal of foreign surface receptors apparently had no consequence.