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Fine Specificity and Cytolytic Activity of Continuously Growing Alloreactive Cytotoxic T Lymphocyte Clones
Author(s) -
SCHAWALLER R.,
RÖLLINGHOFF M.,
WAGNER H.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00012.x
Subject(s) - ctl* , cytotoxic t cell , cytolysis , lytic cycle , effector , t lymphocyte , biology , lysis , monoclonal antibody , cytotoxicity , cloning (programming) , t cell , microbiology and biotechnology , immunology , in vitro , virology , antibody , antigen , immune system , genetics , virus , computer science , programming language
The role of Lyt 1+ T‐cell‐drived secondary CTL inducing factor allowed the cloning of alloreactive cytotoxic T lymphocytes (CTL) by the limiting dilution approach. Several monoclonal cell lines were established in vitro. The lytic activity of some of the cell lines exceeded that of CTL from bulk cultures; that is, 50% of the target cells were lysed at an effector to target cell ratio of 0.04:1. The fine specificity of individual CTL clones is discussed.