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Concanavalin‐A‐binding Proteins on the Surface of Human Malignant and Normal Lymphocytes
Author(s) -
NILSSON S. F.,
EDELSON R.,
MANN D.,
GREEN I.,
WAXDAL M. J.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb03275.x
Subject(s) - concanavalin a , antigen , microbiology and biotechnology , glycoprotein , affinity chromatography , beta 2 microglobulin , cell , biology , antibody , polyacrylamide gel electrophoresis , human leukocyte antigen , chemistry , immunology , biochemistry , enzyme , in vitro
The concanavalin‐A‐binding cell surface glycoproteins from normal and certain leukaemic human lymphocytes were radiolabelled and then solubilized with detergent, isolated by affinity chromatography on Con A insolubilized on agarose beads, and subsequently analysed by SDS‐polyacrylamide gel electrophoresis. Leukaemic T cells from patients with Sezary syndrome were found to express major concanavalin‐A‐binding glycoproteins on their outer surface similar to those of normal T lymphocytes. Leukaemic B cells from patients with chronic lymphocytic leukaemia expressed C on‐A‐binding proteins similar to those of B‐cell lines. HLA antigens were predominant among the major Con‐A‐binding proteins on the surface of the normal and the malignant T cells studied. Human la‐like antigens, HLA antigens, and the cell surface immunoglobulins IgD and IgM represented the major Con‐A‐binding proteins on the B cells studied. β 2 ‐microglobulin was found associated with HLA antigens on both leukaemic and non‐leukaemic T and B cells. The presence of additional Con‐A‐binding proteins expressed on the surface of the different cell types studied is discussed along with some physical characteristics of the human la‐like antigens isolated.