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Degradation of Thymidine to Thymine by Rheumatoid Arthritis Synovial Tissue Eluate
Author(s) -
NYKÄNEN P. J.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb03070.x
Subject(s) - thymidine , thymine , stimulation , rheumatoid arthritis , phytohaemagglutinin , chemistry , synovial fluid , dna , lymphocyte , elution , microbiology and biotechnology , biochemistry , medicine , immunology , endocrinology , chromatography , biology , pathology , alternative medicine , osteoarthritis
A study was made of the effect of rheumatoid synovial tissue eluate on phylohaemagglutinin (PHA) stimulation of peripheral Mood lymphocytes. It was found that rheumatoid synovial tissue eluale caused marked inhibition of 3 H‐thymidine incorporation by lymphocytes stimulated with PHA. Synovial tissue from traumatic joints had no effect. The inhibitor was a heat‐sensitive and nondialysable substance. The inhibitory effect on PHA stimulation was diminished by thymine. In thin‐layer chromatogruphy it showed the capacity to convert thymidine to thymine. Because ihymine is not incorporated by DNA‐synthesizing cells, this report explains why negative results arc obtained when lymphocyles are stimulated by rheumatoid synovial tissue using 3 H‐thymidine incorporation as an indicator of lymphocyte activation.