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Peanut Agglutinin
Author(s) -
LONDON J.,
PERROT J. Y.,
BERRIH S.,
LAROCHE L.,
NIAUDET P.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb03067.x
Subject(s) - peanut agglutinin , peripheral blood mononuclear cell , ficoll , agglutinin , microbiology and biotechnology , lectin , chemistry , immunology , biology , biochemistry , in vitro
Peanut agglutinin (PNA) has previously been shown to be a ‘marker’ for early T subpopulations in mice. We have investigaied whether it could also be used in the study of human mononuclear cells. 50–60% of human thymocytes have binding sites for PNA. When separated on a discontinuous Ficoll gradient, the PNA‐positive thymocytes are found preferentially in the layers corresponding to the immunoincompetent cells. In the peripheral blood only 5% of the mononuclear cells are PNA‐positive and we have shown that these cells are monocytes. In tonsils 13% of PNA+ cells are found and they are mostly lymphocytes. Thus, PNA is a marker for some T cell subsets present in the thymus and tonsils.