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Demonstration of Rheumatoid Factor Idiotypic Antigens on Peripheral Blood B and T Lymphocytes from Patients with Rheumatoid Arthritis
Author(s) -
DOBLOUG J. H.,
FØRRE Ø.,
NATVIG J. B.,
MICHAELSEN T. E.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb02731.x
Subject(s) - idiotype , antiserum , rheumatoid factor , polyclonal antibodies , antigen , rheumatoid arthritis , immunology , immunoglobulin idiotypes , hemagglutination , immunofluorescence , medicine , indirect immunofluorescence , antibody , lymphocyte , peripheral blood , monoclonal antibody
Antisera were raised against three polyclonal IgM rheumatoid factors (RF). After adequate absorptions, the antisera were rendered idiotype‐specific, as assayed by haemagglutination technique. By using the anti‐idiotype antisera in indirect immunofluorescence on peripheral blood lymphocytes from the patients used as donors for the immunizing RF, it was demonstrated that 3–14% of the lymphocytes were stained, and thus had membrane‐bound structures with idiotypic antigens similar to those of the circulating IgM RF of the same patients. While most of these idiotype‐positive lymphocytes were B lymphocytes, it was demonstrated in one patient that about 7% of the T lymphocytes also had the same idiotypic antigens.