Premium
Effects of Anti‐Guinea‐Pig β 2 ‐Microglobulin Antibodies on Lymphocyte Transformation Induced by Specific Antigens or Mitogens
Author(s) -
LÖGDBERG L.,
CIGÉN R.,
BERGGÅRD I.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb02730.x
Subject(s) - concanavalin a , phytohaemagglutinin , pokeweed mitogen , antigen , ovalbumin , antiserum , biology , stimulation , microbiology and biotechnology , antibody , beta 2 microglobulin , guinea pig , beta (programming language) , lymphocyte , immunology , biochemistry , endocrinology , in vitro , computer science , programming language
The effects of a goat anti‐guinea‐pig β 2 ‐microglobulin antiserum (aβ 2 m) on lymphocyte transformation, induced by specilic antigens or mitogens, were studied, aβ 2 m was found to exert inhibitory effects on antigen stimulation, with three different antigens (purified protein derivative, hen egg‐white lysozyme, and ovalbumin). and on stimulation induced by the ‘T‐cell mitogens’, concanavalin A and phytohaemagglutinin. and the ‘mixed mitogen’, pokewecd mitogen, Stimulation induced by the ‘B‐cell mitogens’, dextran sulphate and bacterial lipopolysaccharide, did not seem to he inhibited to the same extent by aβ 2 m. The inhibitory effects seemed specific, as they were not seen with aβ 2 m that had been absorbed on a column with insolubilized purified guinea‐pig β 2 m nor with normal goat serum. Time studies indicated that the inhibitions started already during the first day of culture.