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V and C Gene Contribution in Creating Anti‐α‐1, 3 Dextran Antibodies in Mice
Author(s) -
BELL C.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb02724.x
Subject(s) - antibody , microbiology and biotechnology , immunoglobulin light chain , dextran , chemistry , balb/c , myeloma protein , biology , immunology , biochemistry , immune system
The light (L) and heavy (H) chain and the idiotypic (Id) composition of the antibody (Ab) plaque‐forming cells (PFC) and serum Ab specific for α‐1, 3 dextran have been characterized in murine strains exhibiting the CH‐lg‐l b to e allotypes and in their F 1 hybrids with lg‐l al , BALB/c prototypes, The Ab response of the Ig‐l b to e mice to the α‐1, 3 dextran was low in the kappa ( K ) L chain class with only a minor, sporadic Ab in the lambda (λ) L chain class discernible after prolonged immunization. Two of a total of sixty‐eight C57BI/6 Ig‐l b mice, in a total of 318 Ig‐l b to e mice tested, exhibited, in late responses, a significantly elevated Ab in the λ L class at both serum and PFC levels, equalling at the PFC level the total non‐specific λ PFC values. An Id analysis showed this λ Ab and the K Ab to lack the Id relatedness to the three BALB/c α‐1, 3 dextran‐binding myeloma proteins (MP) Ab prototypes, J‐558, 104 E, and UPC‐102, exhibited by the λ Id’ Ab of the lg‐l al BALB/c prototypes and their F 1 hybrids with the Ig‐l b to e prototypes. Furthermore, affinity differences could be detected by α‐1, 3 nigerodextrans, PFC inhibition analysis, between the late C57BI/6 anti‐α‐1, 3 dextran λ Id − Ab PFC and the λ Id + Ab PFC of the BALB/c and their F 1 progeny.