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V and C Gene Contribution in Creating Anti‐α‐1,3 Dextran Antibodies in Mice
Author(s) -
BELL C.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb02723.x
Subject(s) - microbiology and biotechnology , antibody , immunoglobulin light chain , alpha (finance) , dextran , lambda , kappa , chemistry , biology , stereochemistry , immunology , biochemistry , medicine , physics , construct validity , nursing , linguistics , philosophy , optics , patient satisfaction
The light (L) and heavy (H) chain and the antigenic, idiotypic (Id) composition of the antibody (Ab) plaque‐forming cells (PFC) and serum Ab specific for α‐1, 3 dextran have been characterized in Ig‐l al BALB/c prototype mice, in Ig‐l a− [30] mice, and in BALB/c congenic and recombinant strains. Four distinct Ab Id specificities were identified by using criteria of Id relatedness to three Id‐distinct, α‐1, 3 dextran‐binding BALB/c myeloma proteins (MP), all associated, in the Ig‐l al mice with the lambda (λ) chains: a major, common IdX in 40–90% of the molecules; three minor, individual IdI in 1–49% of the molecules; and a fifth, Id‐undefined one. These specificities were expressed at the PFC and serum level in the μ and γ isotypes. A minor, kappa ( K ), Id‐negative Ab was discerned only at the PFC level. The Ig‐l a− CBA and C3H mice responded with anti‐α‐1, 3 dextran, K Id‐negative Ab. The BALB/c, congenic strain CB20 (BALB/c Ig‐l b , carrying the C57BI/Ka, Ig‐l b variable H (V H ) gene complement and C H phenotype, made anti‐α‐1, 3 dextran K Id‐negative Ab of the Ig‐l b prototype. The recombinant BAB/14, carrying in the C57Bl/Ka C H ‐Ig‐l b phenotype the BALB/c Ig‐l al V H gene(s) controlling anti‐α‐1, 3 dextran λ Ab responsiveness and Id specificity (V H ‐DEX + ), express the BALB/c λId repertoire.