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In Vitro Sensitization of Human T Lymphocytes to Hapten (TNP)‐Conjugated and Non‐treated Autologous Cells is Restricted by Self‐HLA‐D
Author(s) -
THORSBY E.,
NOUSIAINEN H.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb02721.x
Subject(s) - sensitization , in vitro , priming (agriculture) , immunology , human leukocyte antigen , hapten , antigen , biology , microbiology and biotechnology , biochemistry , botany , germination
By co‐culturing human T lymphocytes with TNP‐treated autologous B lymphocytes and macrophages for 10 days in vitro, Sensitization to TNP‐treated autologous cells could be detected in a proliferative assay. By restimulation with different types of allogeneic cells and with cells from donors compatible or incompatible for the HLA‐ABC or ‐D determinants, results were obtained suggesting that the TNP‐specific response was restricted by the HLA‐D but not the ‐ABC antigens of the autologous priming cells. Furthermore, our experiments demonstrate that T lymphocytes can also be primed against non‐treated autologous cells in vitro and suggest that the HLA‐D determinants may be involved also in this auto‐sensitization.