Premium
Severe Suppression of the B‐cell System has No Impact on the Maturation of Natural Killer Cells in Mice
Author(s) -
GIDLUND M.,
OJO E. A.,
ÖRN A.,
WIGZELL H.,
MURGITA R. A.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb02719.x
Subject(s) - antibody dependent cell mediated cytotoxicity , cytolysis , antibody , biology , heterologous , immunology , natural killer cell , cell , spleen , lymphokine activated killer cell , population , interleukin 21 , microbiology and biotechnology , cytotoxicity , immune system , t cell , medicine , monoclonal antibody , in vitro , biochemistry , environmental health , gene
Mice were treated with a heterologous anti‐IgM serum to obtain B‐cell‐deprived mice. Spleen cells from normal and B‐cell‐deprived mice were tested in three different cytolytic systems: natural killer cells (NK); antibody‐dependent cell‐mediated cytolysis (ADCC) against an NIC‐sensitive tumour, P815; and ADCC against chicken erythrocytes. The impact of administration of an interferon‐inducing NK enhancing agent, Tilorone, was also investigated. Whereas the cell population from B‐cell‐deprived mice was significantly suppressed in antibody‐producing cells, the capacity to function in NK or ADCC was largely unimpaired both before and after administration of Tilorone. Our results would imply that mature B cells play no significant role in either the maturation of the NK cells or the expression of their cytolytic ability. Furthermore, effector cells for both NK and ADCC against antibody‐coated tumour target cells were found to be distinct from those functioning in ADCC against chicken erythrocytes.