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Lymphoblastoid Cell Lines are Polyclonal Activators of Human T Lymphocytes
Author(s) -
BOYLSTON A. W.,
ANDERSON R. L.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb02717.x
Subject(s) - polyclonal antibodies , lymphoblast , human cell , cell culture , microbiology and biotechnology , biology , immunology , genetics , antigen
Following stimulation with autologous or allogeneic lymphoblastoid cell lines (LCL) human T lymphocytes acquire two properties which suggest that LCL act as polyclonal activators. Cytotoxic activity, which has an antigen‐specific component, is produced towards normal lymphocytes and LCL, and the cells become capable of mounting proliferative responses to antigens on human B lymphocytes which have accelerated secondary‐type kinetics. Only weak responses to autologous cells occur. In addition, repeated restimulation with the original LCL leads to a progressive increase in the number of cells in the culture for a period of about 4 weeks. This approach may prove a useful way to grow large numbers of human T lymphocytes for further study.