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Characterization of Human Lymphocytes Bearing Fc Receptors for IgE Isolated from Blood and Lymphoid Organs
Author(s) -
HELLSTRÖM U.,
SPIEGELBERG H. L.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb02709.x
Subject(s) - receptor , immunoglobulin e , biology , antibody , cord blood , immunology , complement receptor , surface immunoglobulin , microbiology and biotechnology , antiserum , fc receptor , b cell , complement system , biochemistry
Human lymphocytes isolated from adult peripheral blood and cord blood, tonsils, adenoids and spleens were analysed for Fc receptors for IgE (Fc E ) by rosette assays. The Fc E + cells were also characterized for their class of surface immunoglobulin (sIg), complement receptors, receptors for sheep erythrocytes (E), and Helix pomatia A haemagglutinin (HP), and their abilities to phagocytoze and adhere. The average number of Fc E + cells was in adult peripheral blood 1.2±0.4%, in cord blood 3.0±1.3%, in tonsils 4.2±5.2%, in adenoids 5.8±4.2%, and in spleens varied from 0.8% to 15.8% among individual patients. Overnight culturing of the lymphocytes under conditions that allowed detection of Fc receptors for IgM (Fc μ ) usually lowered the number of Fc E + cells. Neuraminidase treatment caused no change. Rosette formation was inhibited by IgE myeloma proteins and their Fc fragments, but not by mildly reduced and alkylated and heated (56°C) IgE, indicating that the receptors are specific for the native configuration of the IgE Fc fragment. Double cell surface marker analyses with fluoresceinated F(ab') 2 fragments of purified anti‐μ, δ, and γ antibodies used to label the Fc E rosette‐forming lymphocytes from peripheral adult and cord blood showed that 50–80% were sIgM + but only 0–28% were sIgD + . In contrast, approximately 80% of the Fc E + cells from tonsils, adenoids and spleens were sIgM + and sIgD + . The Fc E + lymphocytes represented 10–20% of the sIgM + lymphocytes in both the peripheral adult and cord blood. Depletion and enrichment experiments indicated that most of the Fc E + cells bear complement receptors. Lymphocytes having both E and Fc E receptors were not found. Furthermore, the lymphocytes with HP receptors, a marker for T cells and immature B cells, were Fc E − . Monocytes and neutrophil granulocytes did not form Fc E rosettes. These data indicate that a minor population of human B lymphocytes have Fc E receptors. The majority of the Fc E + lymphocytes in the blood differ from those in tonsils, adenoids and spleen in that the majority of the former are sIgM + /sIgD − and the latter sIgM + /sIgD + . The sIgM + /sIgD − Fc E + cells in the blood are probably relatively mature lymphocytes since they lacked HP receptors, which are found on immature B cells