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Detection of Antibodies in Human Sera to Streptococcal Groups A and C Carbohydrates by a Radioimmunoassay
Author(s) -
AASTED B.,
BERNSTEIN D.,
KLAPPER D. G.,
KHOLY A.,
KRAUSE R. M.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb02707.x
Subject(s) - carriage , antibody , group a , radioimmunoassay , group b , immunology , incidence (geometry) , medicine , throat , streptococcus , epidemiology , serology , pharynx , biology , bacteria , pathology , surgery , physics , optics , genetics
Human antibodies to Streptococcal groups A and C carbohydrates were measured quantitatively with a radioimmunoassay using tyrosylated 125 I carbohydrate. Analysis of acute and convalescent sera from people with groups A or C streptococcal pharyngeal infection or persistent carriage revealed a significant rise in antibodies. Inhibition reactions with the cold carbohydrate indicated the specificity of the elicited antibodies. In some instances, group C as well as group A antibodies occurred after group A pharyngeal infections. Further clinical and epidemiological studies are required to determine the significance of group‐specific antibodies. Antistreptolysin‐O (ASO) rises were observed in individuals with group C antibody responses after persistent group C pharyngeal carriage. For this reason, epidemiological surveys that rely primarily on ASO surveys to determine the incidence of group A streptococcal infections must be interpreted wilh caution, at least in the developing countries, where group C pharyngeal carriage is common.