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The Fc Binding Regions in Protein A are not Responsible for the Polyclonal B Cell Activating Property of Protein A
Author(s) -
SJÖDAHL J.,
MÖLLER G.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb01394.x
Subject(s) - polyclonal antibodies , activator (genetics) , antibody , chemistry , in vitro , microbiology and biotechnology , monomer , binding protein , biochemistry , biology , receptor , immunology , gene , organic chemistry , polymer
To test whether the polyclonal B cell activating property of protein A is due to its ability to bind to the Fc part of immunoglobulins, experiments were carried out with purified monomeric or dimeric Fc‐binding fragments of protein A. Neither monomeric nor dimeric fragments induced polyclonal antibody synthesis in vitro, whereas intact protein A was a strong activator. It is concluded that the Fc binding part of protein A is not responsible for activation of polyclonal antibody synthesis.