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Cell‐mediated Cytotoxicity during Vaccinia Virus Revaccination in Man: Influence of Antibodies and Interferon
Author(s) -
MØLLERLARSEN A.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb01388.x
Subject(s) - cytotoxicity , vaccinia , virology , antibody , virus , interferon , antibody dependent cell mediated cytotoxicity , immunology , vaccination , biology , microbiology and biotechnology , in vitro , biochemistry , recombinant dna , gene
Seven healthy human adults were revaccinated with vaccinia virus. Cell‐mediated cytotoxicity to vaccinia virus‐i nfected fibroblasts was investigated on the day of vaccination and on days with peak activity. Three donors were studied until day 30 after vaccination. The addition of interferon to cytotoxicity reactions resulted in an increase in killing. This increase was not seen when antibodies were added. When a mixture of lymphocytes from a revaccinaied and a non‐rcvaccin‐ated donor was used as effector cells, the killing observed corresponded to the killing seen with lymphocytes from the revaccinaied donor, when tested alone. This finding indicates that no antibodies or other soluble mediators capable of increasing cytotoxicity are released from the lymphocytes during the cytotoxicity assay.