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Heating at 56 ° C does not Eliminate Immune Complex‐bound Clq
Author(s) -
TEPPO A.M.,
WAGER O.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb01372.x
Subject(s) - chemistry , centrifugation , immune system , albumin , biochemistry , biophysics , immunology , biology
The effect of heating at 56°C on free and complex‐bound human Clq was studied by radioisotope and sucrose gradient centrifugation techniques. The ability of soluble complexes of egg albumin, rabbit anti‐egg albumin, and human Clq to bind extrinsic iodinated Clq did not increase following heating at 56°C. The results of this study did not support the postulated release of complex‐bound Clq on healing at 56°C. They also showed that free Clq was more heat‐labile than Clq in the Clqrs state. The importance of elimination or monitoring of intrinsic Clq in Clq assays for circulating immune complexes is stressed.