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The Effect of Specific Antibody on the Generation of Cytotoxic T Lymphocytes and the Recovery of Mice from Influenza Virus Infection
Author(s) -
YAP K. L.,
ADA G. L.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb01358.x
Subject(s) - virus , antibody , cytotoxic t cell , virology , spleen , nasal administration , biology , titer , infectious dose , inoculation , immunology , in vitro , biochemistry
A study has been made of the effect of humoral antibody on the generation of specific cytotoxic T cells (Tc) in the spleen or lungs after intravenous injection or intranasal inoculation of infectious influenza virus. Antibody injected before or at the same time as virus inhibited completely the generation of Tc in the spleen. If injected 1 h after virus, the inhibition was reduced by 50% and little inhibition occurred if antibody was injected 6 h after virus. This suggested that antibody failed to influence Tc generation once infection of stimulating cells had occurred. Antibody injected intravenously 24 h after intranasal inoculalion of virus into normal mice did not affect the level of cytotoxic activity present in the lungs, and tracc amounts only (> 1 log 10 EID 5–0 ) could be recovered from the lungs at 6 days. As there is a high litre (>6 log 10 EID 5–0 ) of infectious virus in the lungs of mice 24 h after infection, this represents a very efficient control mechanism. The same protocol carried out with athymic mice gave only a partial clearance (e. 3 log 10 EID 50 ) of virus in the lungs. It was suggested that a major role of humoral antibody was to limit infection by the virus, and in this respect it complemented the action of Tc.