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Plaque Assay with Protein A‐coated Erythrocytes for the Evaluation of Human Immunoglobulin‐secreting Cells Induced by Pokeweed Mitogen
Author(s) -
LANZAVECCHIA A.,
SACCHI G.,
MACCARIO R.,
VITIELLO A.,
NESPOLI L.,
UGAZIO A. G.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb01354.x
Subject(s) - polyclonal antibodies , pokeweed mitogen , antiserum , in vitro , antibody , virus quantification , microbiology and biotechnology , biology , immunoglobulin g , immunoglobulin m , immunology , peripheral blood mononuclear cell , biochemistry , virus
Cells secreting IgM, IgG and IgA were evaluated in cultures of human peripheral blood lymphocytes stimulated in vitro with pokeweed mitogen by a haemolytic plaque assay using protein A‐coated erythrocytes in the presence of class‐specifie antisera. Kinetic study revealed that immunoglobulin‐secreting cells appeared after 3 days of culture and peaked between days 5 and 7. IgM‐secreting cells predominated throughout the culture period. This plaque assay is a useful and sensitive in vitro test for the evaluation of polyclonal B‐cell activation in humans and may provide a good approach to the study of disturbances in the synthesis of the various classes of immunoglobulins.