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HLA‐D‐restricted Antigen Activation of Sensitized T Lymphocytes: Studies on the Ability of HLA‐D/DR‐expressing B Lymphocytes to Substitute for Macrophages in Antigen Activation
Author(s) -
BERGHOLTZ B. O.,
THORSBY E.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb01349.x
Subject(s) - immunology , human leukocyte antigen , antigen , lymphocyte activation , t lymphocyte , biology , immune system , t cell
The proliferative response of sensitized human T lymphocytes to purified protein derivative (PPD) and to trinitrophenyl (TNP)‐conjugated autologous cells in vitro is restricted by self HLA‐D/DR determinants. Here we report that the PPD‐specific response is strictly related to the content of phagocytosing celts (macrophages, M) in the cultures and that an optinal PPD response occurred at a T/M ratio between 10:1 and 5:1. B‐cell‐enriched suspensions, which also express the HLA‐D/DR determinants, were not able to replace the macrophages in this HLA‐D/DR‐restricted response. On the other hand, TNP‐trealed similarly prepared B cells were in most instances effective in inducing a secondary TNP‐specific response of in vitro‐sensitized T cells.