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Rat Memory T Lymphocytes
Author(s) -
BURCKHARDT J. J.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb01344.x
Subject(s) - phytohaemagglutinin , in vitro , antigen , in vivo , microbiology and biotechnology , t cell , spleen , macrophage , chemistry , immunology , biology , mitogen activated protein kinase , t lymphocyte , immune system , biochemistry
Protein‐coated silica, a macrophage toxin, was used to assess the requirement for accessory cells in the induction of an in vitro proliferative response to (i) antigens from Actinomyces viscosus and (ii) the mitogens conconavalin A (Con A) and phytohaemagglutinin (PHA). T cells were obtained from RIC‐Sprague‐Dawley rats primed in vivo with A. viscosus Nyl by splenectomy and filtering the spleen cell suspensions through Degalan Ig‐anti‐IgG columns. In the presence of 100 mTg silica/ml during 4 days of culture, the proliferative response of T lymphocytes was not diminished. In contrast, when the T cells were precultured with silica for 24 h, washed, and subsequently cultured with the antigen fractions, antigen‐induced proliferation was abolished. This procedure, however, had no influence on mitogen‐induced T‐cell activation. It is therefore concluded that the antigen‐dependent anamnestic in vitro response (but not activation by mitogens) of rat T lymphocytes needs help from silica‐sensitive macrophages.