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Demonstration of Anti‐Rubella Antibody‐secreting Cells in Rheumatoid Arthritis Patients
Author(s) -
CHATTOPADHYAY H.,
CHATTOPADHYAY C.,
NATVIG J. B.,
WIGER D.,
MELLBYE O. J.
Publication year - 1979
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1979.tb01333.x
Subject(s) - virology , antigen , antibody , rubella , mumps virus , medicine , virus , immunology , rubella virus , paramyxoviridae , measles virus , cytomegalovirus , measles , viral disease , herpesviridae , vaccination
In the present study we describe a plaque‐forming cell assay using erythrocytes coated with viral antigen, which detected anti‐viral antibody‐secreting cells against various viral antigens. These anti‐viral antibody‐secreting cells were studied in normal individuals with known viral infections and in rheumatoid arthritis patients. Rubella anti‐viral antibody‐secreting cells were present after induction in the peripheral blood of eight out of ten patients. No plaques were seen before induction. Synovial tissue of seven patients out of ten showed rubella‐antigen‐specific plaques before induction. In all three patients tested, the numbers of plaques increased after induction. The peripheral blood of only one patient showed plaque‐forming cells against mumps virus and cytomegalovirus (CMV) antigen. No other patients showed any plaques against CMV, respiratory syncytial virus, mumps virus, measles virus, adenovirus, and varicella zuster virus antigens. The method appears to he promising in studying viral antibody‐secreting cells in human immuno‐pathology.