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Light and Electron Microscope Immunolocalization of Alpha‐fetoprotein in Rat Liver Cells in vivo and in vitro
Author(s) -
GUILLOUZO A.,
BÉLANGER L.,
BEAUMONT C.,
BRIGGS R.,
CHIU J. F.,
FLEISCHER B.,
FLEISCHER S.,
VALET J. P.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb03934.x
Subject(s) - golgi apparatus , albumin , endoplasmic reticulum , immunoperoxidase , electron microscope , biology , hepatocyte , in vitro , secretion , liver cytology , pathology , endocrinology , microbiology and biotechnology , biochemistry , medicine , immunology , antibody , liver metabolism , physics , optics , monoclonal antibody
In neonatal rat liver, AFP is localized only in typical hepatocytes. Their lobular distribution changes throughout the neonatal period. Some AFP+ cells also contain albumin. Less than 5% of AFP+ cells incorporate 3 H‐thymidine (2‐hour pulse). Injections of dexamethasone suppress AFP positivity but not albumin positivity nor 3 H‐thymidine incorporation. AFP is also localized in typical hepatocytes in newborn rat isolated liver cells or liver explants in culture. During the early phase of AFP induction in rats, by mDAB feeding, AFP is detected in cells smaller than the normal hepatocytes and preferentially situated in periportal areas of the liver. Electron microscope immunoperoxidase localizations in newborn rat liver show that AFP is present on bound ribosomes, in the lumina of rough and smooth endoplasmic reticulum, and in the Golgi apparatus. Direct AFP measurement on isolated organelles confirmed this distribution. It indicates a synthesis and secretion pattern similar to that of albumin.