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Suppression of in vitro Parameters of the Cell‐mediated Immunity by Human Alpha‐1‐fetoprotein
Author(s) -
AUER J. O.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb03932.x
Subject(s) - sephadex , concanavalin a , size exclusion chromatography , microbiology and biotechnology , in vitro , chemistry , biology , biochemistry , enzyme
A possible immunoregulatory role of human alpha‐1‐fetoprotein (HAFP) was investigated, since in contrast to mouse AFP such function has been denied for HAFP. HAFP enriched fractions as well as pure HAFP were obtained by means of two different procedures. After passage of HAFP containing ascites of patients with primary liver carcinoma on an anti‐HAFP immunosorbent column, the retained proteins were eluted first by a glycine‐NaOH‐buffer (HAFP 1) and second by NaSCN (HAFP II). HAFP was further purified by passage of the HAFP containing fractions on an anti‐whole human serum immunosorbent column, which resulted in semipurified HAFP I and II. Pure HAFP was obtained by recycling on the anti‐whole‐human serum immunosorbent column as well as by final Sephadex G‐100 gel filtration. At concentrations of 150–200 μg/ml, only HAFP I, semipurified as well as pure, exerted consistently a profound suppressive effect on the mixed leukocyte reaction as well as on the Concanavalin A induced lymphocyte proliferation, while it usually failed to do so in PHA stimulated lymphocyte cultures. At concentrations which were immunosuppressive, pure HAFP I showed no influence on the proportion of E‐rosetting lymphocytes. HAFP II did not exert comparable immunoregulatory effects.