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Rat Yolk Sac Tumor: Biological Aspects and Microheterogeneity of AFP
Author(s) -
SAKASHITA S.,
TSUKADA Y.,
HIRAI H.,
TSUJI I.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb03919.x
Subject(s) - yolk sac , ascites , albumin , neuraminidase , immunoelectrophoresis , sialic acid , in vitro , bovine serum albumin , biology , pathology , medicine , endocrinology , chemistry , biochemistry , antibody , immunology , embryo , microbiology and biotechnology , enzyme
α‐Fetoprotein (AFP)‐producing yolk sac tumors were established in rats as transplantable tumor lines growing in either solid or ascitic form. AFP levels in the ascites and in the serum of recipients were quite high, but higher in castrated female rats than male rats. The tumor cells were cultured also in vitro and showed synthesis of AFP and albumin. AFP produced by the yolk sac tumors was investigated comparatively with AFP produced by a hepatoma. There was no remarkable difference between two AFPs in behavior in gel filtration; however, AFP of yolk sac tumors migrated more slowly than that of hepatoma in immunoelectrophoresis. However, the mobilities of two AFP preparations became same after desialization by neuraminidase treatment. This fact suggests that AFP of yolk sac tumors contains sialic acids in smaller amount than that of hepatomas.