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Immunofluorescent Demonstration of Alpha‐fetoprotein in Tissue Culture from Human Endodermal Sinus Tumors, Rat Yolk Sac Carcinoma and Fetal Rat Yolk Sac
Author(s) -
ALBRECHTSEN R.,
HIRAI H.,
LINDER D.,
NØRGAARDPEDERSEN B.,
WEWER U.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb03916.x
Subject(s) - yolk sac , endoderm , alpha fetoprotein , endodermal sinus tumor , biology , fetus , pathology , staining , antiserum , embryo , anatomy , cellular differentiation , medicine , hepatocellular carcinoma , microbiology and biotechnology , antibody , immunology , pregnancy , cancer research , biochemistry , genetics , gene
An in vitro model for study of AFP (alpha‐fetoprotein) synthesis is presented using immunofluorescent microscopic examination of cultured cells from rat yolk sac, experimental rat yolk sac carcinoma and human endodermal sinus tumors (EST). The presence of positive staining for AFP in all cases by use of specific rabbit antisera against rat and human AFP supports the concept that AFP synthesis in germ cell tumors is confined to the yolk sac endoderm. The variation of positive staining cells due to a variable protein synthesis is discussed.