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Demonstration of High‐Affinity Binding Sites for C3a Anaphylatoxin on Guinea‐Pig Platelets
Author(s) -
BECKER S.,
HADDING U.,
SCHORLEMMER H. U.,
BITTERSUERMANN D.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00555.x
Subject(s) - receptor , platelet , guinea pig , serotonin , anaphylatoxin , 5 ht receptor , chemistry , cell surface receptor , biophysics , biology , microbiology and biotechnology , biochemistry , endocrinology , immunology , complement system , antibody
3 H‐serotonin release from guinea‐pig platelets was demonstrated to be the consequence of C3a binding to these cells. A Scatchard analysis of dose‐response data of the 125 I‐C3a binding pattern to guinea‐pig platelets pointed to the existence of binding sites with high and low affinity for the C3a molecule (HA and LA receptors). HA receptors are specific for C3a with intact C‐terminal arginine. whereas C3a desarg only interacts with LA receptors. The release of serotonin may be induced by a combined reaction of C3a with HA receptors and LA receptors on the platelet membrane.