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Association of Mouse Major Histocompatibility and Rauscher Murine Leukaemia Virus Envelope Glycoprotein Antigens on Leukaemia Cells and their Recognition by Syngeneic Virus‐Immune‐Cytotoxic T‐Lymphocytes
Author(s) -
ZARLING D. A.,
KESHET I.,
WATSON A.,
BACH F. H.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00549.x
Subject(s) - antigen , virology , cytotoxic t cell , biology , antiserum , rickettsia , murine leukemia virus , immune system , microbiology and biotechnology , virus , balb/c , titer , immunology , in vitro , biochemistry
Physical association was measured between MLV gp70, the envelope glycoprotein of Rauscher murine leukaemia virus (R‐MLV), and serologically defined H‐2 antigens on the surface of R‐MLV transformed C57BL/6 (H‐2D b k b ) mouse leukaemia cells (RBL‐5A). Capping and patching with antisera against H‐2D b caused specific co‐capping and co‐patching of R‐MLV gp70 antigens as seen by fluorescence microscopy. Despite the physical proximity of R‐MLV gp70 and H‐2D b antigens, high titre αR‐MLV gp70 sera had no effect in blocking syngeneic T‐lymphocyte mediated cytolysis of RBL‐5A cells whereas αaH‐2D b sera were effective.