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Polyclonal Activation of Human B Lymphocytes in Vitro by Pokeweed Mitogen: a Simple Technique for the Simultaneous Assessment of Cell Proliferation, Generation of Plasma Cells, Plaque‐forming Cells and Immunoglobulin Production
Author(s) -
NESPOLI L.,
VITIELLO A.,
MACCARIO A. R.,
LANZAVECCHIA A.,
UGAZIO A. G.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00548.x
Subject(s) - pokeweed mitogen , polyclonal antibodies , fluorescein isothiocyanate , antibody , in vitro , biology , microbiology and biotechnology , mitogen activated protein kinase , polyclonal b cell response , immunology , peripheral blood mononuclear cell , b cell , biochemistry , fluorescence , physics , b cell receptor , quantum mechanics
A simple technique has been worked out for the simultaneous assessment of cell proliferation, generation of plasma cells, plaque‐forming cells and immunoglobulin production from single cultures of 1 × 10 4 human peripheral blood lymphocytes stimulated with pokeweed mitogen (PWM). Kinetic studies showed that number of cells, thymidine incorporation rate, number of plaque‐forming cells against fluorescein‐isothiocyanate‐haptenated sheep erythrocytes and concentration of IgM and IgG in the supernatant peaked on the average between day 5 and 7 of culture. This technique is particularly suitable for the analysis of hypogammaglobulinaemias in infancy.