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Lymphocyte Associated β 2 ‐Microglobulin Studied by Crossed Radioimmunoelectrophoresis
Author(s) -
PLESNER T.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00530.x
Subject(s) - antiserum , beta 2 microglobulin , immunoelectrophoresis , lymphocyte , incubation , microbiology and biotechnology , antibody , electrophoresis , chemistry , absorption (acoustics) , biology , chromatography , biochemistry , immunology , physics , acoustics
A sensitive radioimmunoelectrophoretic assay was employed in the study of β 2 ‐microglobulin (β 2 ) from human blood lymphocytes, allowing the detection of 2.5 pmol β 2 m corresponding to 3 × 10 6 lymphocytes. The intrinsic (amphiphilic) membrane proteins were solubilized and examined in crossed immunoelectrophoresis against rabbit antisera in the presence of non‐ionic detergent. The β 2 m associated precipitate was traced by post‐electrophoretic incubation with 125 I‐labelled antibodies to β 2 m and autoradiography, A polyspecific rabbit antiserum to human lymphoid cells retained its capacity to precipitate lymphocyte β 2 m after absorption with isolated (on an immunosorbent column), showing that lymphocyte β 2 m is complexed to other molecules. No free β 2 m was found on lymphocytes when examined against the absorbed anti‐serum to human lymphoid cells in an intermediate gel and anti‐β 2 m in a reference gel.