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A Chemical Approach to the Mechanism of B‐Lymphocyte Activation
Author(s) -
VIDALGOMEZ J.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00525.x
Subject(s) - hapten , immunogenicity , dinitrophenyl , conjugate , lysine , lymphocyte , chemistry , ficoll , cellulose , immune system , lipopolysaccharide , antigen , microbiology and biotechnology , biochemistry , immunology , antibody , biology , amino acid , in vitro , peripheral blood mononuclear cell , mathematical analysis , mathematics
Dinitrophenyl (DNP)‐lysine‐polymethylmethacrylate and DNP‐cellulose conjugates do not irreversibly inactivate anti‐DNP antigen‐sensitive cells, regardless of the dose (up to 10 mg) or persistence of the stimulation (up to 2 weeks). Since these conjugates constitute pure hapten presentations, it is concluded that the pure hapten presentation to B lymphocyte does not irreversibly Inactivate them. When murine spleen cells are cultured with Escherichia coll lipopolysaccharide (LPS) and (non‐immunogenic) DNP‐lysine‐polymethylmethacrylate or (non‐immunogenic) DNP‐cellulose conjugates. an anti‐DNP immune response occurs. However, replacement of DNP‐lysine‐polymethylmethacrylate with polymethylmethacrylate, or DNP‐cellulose with cellulose, also results in a similar anti‐DNP response. It is consequently concluded that the anti‐DNP responses are entirely elicited by LPS, the hapten DNP being inoperative. The anti‐DNP response elicited by DNP‐Ficoll is, upon exhaustive testing, carrier‐dependent This implies that the mechanism of DNP‐Ficoll immunogenicity is not two cooperative signals passed on to B lymphocytes via the hapten DNP. These results argue against any two‐signal model of B‐lymphocyte activation.