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Lymphocyte Subpopulations in Man: Ox Erythrocytes as Indicators in the EA‐ and EAC‐Rosette Tests: Serological and Technical Aspects
Author(s) -
JOHNSEN H. E.,
MADSEN M.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00517.x
Subject(s) - serology , antibody , lymphocyte , immunology , sensitization , antiserum , rosette (schizont appearance) , complement (music) , zymosan , biology , microbiology and biotechnology , chemistry , biochemistry , in vitro , phenotype , complementation , gene
Serological properties of ox erythrocytes (ORBC) make it possible to select cells which exhibit weak agglutinability despite strong antibody sensitization. This property and the non‐binding of unsensitized ORBC to lymphocyte surface membranes make these cells excellently suited as indicators in techniques for the identification of erythrocyte‐antibody (EA) and erythrocyte‐antibody‐Complement (EAC) rosette‐forming lymphocytes (RFC). This report describes the relevant serology for the selection of appropriate cells and antisera. Further, some of the technical aspects of these tests are discussed. A simple method for the sensitization of ORBC with complement is described. The basis for this method is the naturally occurring complement‐binding anti‐ORBC antibodies of the IgM class in human sera. After zymosan treatment the sera are deficient in the fifth component of complement and hence non‐haemolytic, which make these sufficient as sensitizing agents in die preparation of EAC indicator cells. The relations of the FA‐and EAC‐RFC to be established T and B lymphocyte subpopulations are revealed by the enrichment and depletion of lymphocytes rosetting with 2‐aminoethylisothiouronium bromide (AET)‐treated sheep erythrocytes (SRBC).