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HLA‐D Restriction of the Macrophage‐Dependent Response of Immune Human T Lymphocytes to PPD in Vitro: Inhibition by Anti‐HLA‐DR Antisera
Author(s) -
BERGHOLTZ B. O.,
THORSBY E.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00496.x
Subject(s) - human leukocyte antigen , in vitro , antiserum , immunology , immune system , macrophage , biology , antigen , genetics
The response of T cells from sensitized individuals to low doses of PPD in vitro is macrophage‐dependent. By testing different allogeneic combinations of macrophages and T lymphocytes, it was found that an optimal response required that antigen be presented by macrophages sharing at least one of the HLA‐D determinants of the T cell donor. Antisera recognizing HLA‐A, ‐B or ‐DR antigens were found to be able to inhibit this proliferative response. The anti‐HLA‐DR antisera were found to exert their inhibitory effect only when directed towards an antigen shared by the donors of the T lymphocytes and the macrophages. Anti‐HLA‐A and ‐B sera, however, had an inhibitory effect when reactive with the responding T lymphocytes, irrespective of their reactivity with the cooperating macrophages. It is concluded that an optimal secondary response of in‐vivo‐immunized T lymphocytes to PPD requires the combined recognition of the antigen and ‘self’ membrane structures encoded by the HLA‐D locus.