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Possible Detection of HLA‐DR Alloantigenic Specificities in Man with Unabsorbed Rabbit Antisera
Author(s) -
SOLHEIM B. G.,
FUKS A.,
SMITH L.,
STROMINGER J. L.,
THORSBY E.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00491.x
Subject(s) - antiserum , precipitin , cytotoxic t cell , antibody , biology , microbiology and biotechnology , antigen , cytotoxicity , human leukocyte antigen , typing , rabbit (cipher) , immunology , biochemistry , in vitro , statistics , mathematics
Thirty‐six rabbits were immunized with precipitin lines formed between detergent solubilized membrane fractions from HLA‐D‐homozygous cells and a rabbit antiserum to human B cells (anti‐p 23/30). Thirty‐one of the rabbits produced B‐cell specific cytotoxic antibodies and twelve of the antibodies were also precipitating in gel diffusion. In cytotoxicity tests six of the antisera showed clear preferential reactivity with the immunizing HLA‐D (DR) antigen. Thus immunizazation of rabbits might prove valuable for the production of HLA‐DR typing reagents.