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Demonstration of Electrophoretically Restricted Virus‐Specific Antibodies in Serum and Cerebrospinal Fluid by Imprint Electroimmunofixation
Author(s) -
NORDAL H. J.,
VANDVIK B.,
NORRBY E.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00468.x
Subject(s) - antibody , cerebrospinal fluid , antigen , virology , mumps virus , virus , subacute sclerosing panencephalitis , measles virus , herpes simplex virus , biology , measles , immunology , vaccination , neuroscience
A sensitive technique for the electrophoretic characterization of virus‐specific antibodies is described, Electrophoretically separated Ig is allowed to diffuse into a virus‐antigen containing gel. The antibodies hound to viral antigen are then demonstrated by 125 I‐labelled rabbit anti‐human Ig and autoradiography. Electrophoretically restricted antibodies against measles, rubella, mumps or herpes simplex viruses were demonstrated in some normal sera. The antibody patterns of normal cerebrospinal fluids (CSF) closely resembled those of the matching sera. A selective increase of oligoclonal antibodies was demonstrated in CSF from patients with infection of the central nervous system (CNS) caused by any of the four viruses. We propose that the method may be used to demonstrate local synthesis in the CNS of antibodies against viral or other antigens.