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Effect of in Vivo Exposure to Allogeneic Cells upon Subsequent in Vitro T Cell Responses and upon Allograft Rejection
Author(s) -
COOLEY M. A.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00467.x
Subject(s) - spleen , sensitization , in vivo , cytotoxic t cell , in vitro , immunology , lymph node , mixed lymphocyte reaction , lymph , lymphatic system , lymphocyte , cell , t cell , medicine , biology , pathology , immune system , biochemistry , microbiology and biotechnology , genetics
The effect of intravenous injection of 10 8 BALB/c spleen cells into C57B1/6J recipients was assayed by both mixed lymphocyte culture (MLC) of recipient lymphocytes, and by grafting donor (BALB/c) thyroids into recipient mice. It was observed that a single intravenous injection produced depression of proliferative and cytotoxic T cell responses in MLC of spleen, lymph node and peripheral blood lymphocytes of the recipients. This effect was specific for the sensitizing genotype (MLC of recipient und third‐party C8A/H lymphocytes was unaffected), and persisted for several days after sensitization. The pattern of this diminished response suggested that the effect was due to a combination of recruitment of reactive lymphocytes from peripheral lymphoid populations, and the generation of alloantigen (H‐2?)‐specific suppressor T cells in the spleen. In contrast to these findings in vitro, a similar sensitization led only to accelerated rejection of thyroid allgrafts in vivo.