Premium
The Affinity of Soluble Immune Complexes for Concanavalin A
Author(s) -
HEIMER R.,
KLEIN G.
Publication year - 1978
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1978.tb00459.x
Subject(s) - affinity chromatography , immune system , sepharose , concanavalin a , antibody , antigen , immune complex , chemistry , immunology , biology , microbiology and biotechnology , biochemistry , enzyme , in vitro
Con A‐Sepharose affinity chromatography may be used in the analysis and classification of immune complexes. Experiments with model immune complexes suggest that the degree of affinity of an immune complex for Con A‐Sepharose is determined by the antigen rather than the IgG antibody of the complex. It is possible that partial characterization of unknown antigens linked to IgG in immune complexes may be achieved in many diseases. Preliminary explorations with selected human sera indicate that the IgG containing immune complexes in Burkitt's lymphoma and nasopharyngeal carcinoma have affinity for Con A‐Sepharose. By contrast IgG containing immune complexes in chronic hepatitis B seem to lack affinity for Con A‐Sepharose.